To your chromosome 4, the three QTLs were from the 66
Package plots indicating the effects of one’s marker genotypic kinds (AAAA, AAAB, AABB, ABBB, BBBB) off cuatro applicant SNPs on tuber glucose attention (GLU); bud-end fry color (FBE); stem-tuber fry color (FSE); ID, Idaho
The latest co-localization of several QTLs within maturity locus region inside investigation is consistent with the observed patterns out of phenotypic correlations. 01) with several characteristics overlapping a comparable hereditary status (Fig. 1). As an example, EBID11 and you may VWID11 results each other presented a strong bad relationship with MAT95ID and you will MAT120ID. So it trend may give an explanation for biggest effect QTL having later readiness closely in the put off start of VW and you will EB, or simply, very early readiness resulting in very early onset of VW and you may EB. A relatively highest correlation has also been seen between vine maturity and growth routine, which have very early maturity on the significantly more distribute gains habit. Alternatively, a low correlation (roentgen ? 0.2) between vine maturity and you will one another tuber glucose attention and bud-prevent fry colour is actually consistent with the latest models of to your QTL consequences.
Tuber sugar, bud-prevent and you may base-end fry colour
Plus chromosome 5, significant QTLs to own sugar, bud-avoid and you may base-stop fry color have been sensed within the overlapping regions of chromosomes cuatro, 6, ten, and you may 11. 0 cM to the LOD ratings ranging from cuatro.five to six.0 (Table step three, Fig. 4). These types of QTLs explained ranging from seven.2 and you may 11.3% of your own attribute variance. Investigation of various effortless activities expressed an individual most useful-fitting design for everyone about three characteristics, that have a two fold-simplex allele (AAAB ? AAAB) towards the homologous chromosomes H2 and you will H5 out-of RGR and Publicity, respectively (Table 3, Additional document 8: Shape S4). To have sugar, brand new B allele was with the large sugar focus, when you are to have bud-prevent and you may base-stop fry color, the presence of the newest B allele indicated down reflectance thinking (Fig. 3b).
LOD score shipping out-of QTLs for three qualities over the twelve potato chromosomes (Chr). Abbreviations was such as Fig. 1
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this die beliebtesten thailändischen Dating-Seiten SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
7 Mb) which includes multiple family genes in the starch/glucose inter-sales and hexokinase (HXK-6, PGSC0003DMG400016521), fructokinase (FRK-6/step one, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and invertase (INV-na-6/step 1, PGSC0003DMG400026107, INV-6/2, PGSC0003DMG400033142) genes.