Embolon, Thessaloniki, was basically characterized as to the mode out of reproduction and ploidy
BOWEN, S.T., DURKIN, J.P., STERLING, Grams. and you will CLARK, L.S. (1978) Artemia hemoglobins: hereditary variation in the parthenogenetic and zygogenetic populations. Bull., 155, 273287.
Cysts from parthenogenetic Artemia challenges amassed on Citros and you will Meters. Embolon saltworks into the Northern Greece are analyzed due to their prospective fool around with in aquaculture. Next characterizations was indeed did: tumefaction and you can naupliar biometrics, tumefaction hatching functions, greasy acidic reputation of your own nauplii, caloric content regarding nauplii held from the twenty-five° C plus in a fridge (cuatro 8° C). These investigations suggests that both Artemia stresses learnt display an effective features to be used inside the aquaculture, especially in culturing fresh-water varieties. Brand new biometrical analysis off cysts, nauplii and people suggests a top standard of resemblance along with other parthenogenetic stresses out-of various geographical present, but particularly which have tetraploid Artemia of Spain. Brand new Greek Artemia strains can not be considered as ‘present to own aquacultural uses unless of course best management of brand new saltworks try in hopes.
Because brine shrimp Artemia is out-of higher financial pros, you will need to evaluate new ‘industrial qualities of numerous communities for its possibility of software for the aquaculture. When you look at the early in the day degree, a few Northern Greek Artemia communities utilized in Citros, Piera and you will Meters. These people were seen to be parthenogenetic and you can tetraploid (Abatzopoulos et al., 1986). Analysis of degree on DNA reassociation Kinetics as well as on thirteen enzymatic possibilities of these two communities imply that they fall under you to kinds (Abatzopoulos et al., 1987).
Biol
Inside paper we breakdown of the brand new biometrical characteristics out of cysts, nauplii and grownups, to the hatching qualities out-of cysts, we.age. hatching rates, hatching percentage, hatching performance and you may hatching output, on the alterations in lifeless weight and you can caloric blogs out of instar-We nauplii managed during the room temperature and you can cold storage criteria, and on the latest fatty acid profiles out-of instar-We nauplii.
Both communities out of North Greek Artemia examined come from new saltworks regarding Citros, Pieria (C filters), and regarding the saltworks of Yards. Embolon, Thessaloniki (Me personally strain). Cysts have been built-up during the 1983, canned based on Sorgeloos mais aussi al. (1978), and stored in closed synthetic handbags within -25° C, to possess upcoming fool around with.
Adult brine shrimps was indeed anesthezied from inside the chlorofrom-over loaded and you may mentioned less than a dissection microscope playing with a beneficial Leitz micrometer, The following measurements was indeed performed : complete size, intestinal duration, maximum depth out of brood pouch, thickness out-of 3rd abdomianal phase, thickness regarding lead, period of basic antenna, maximal diameter off cutting-edge eye, and you may point anywhere between cutting-edge vision (Amat, 1980). Dimensions was did on the animals – test proportions = 50 animals-coming from crazy cysts and you will cultured not as much as fundamental labouratory requirements (yeast once the restaurants, temperatures out-of twenty-five + 1° C, and you will seawater of thirty-five% salinity). The information and knowledge had been mathematically treated from the investigation out of covariance (Sokal Rohlf https://datingranking.net/nevada-dating/, 1981).
Hatching analyses was achieved according to Vanhaecke Sorgeloos (1983). Hatching results, hatching percentage, hatching rates and hatching efficiency was indeed analysed adopting the rates and you will hatching returns had been analysed pursuing the Sorgeloos ainsi que al. (1978), Bruggeman et al. (1980), Vanhaecke Sorgeloos (1982), and you will Vanhaecke Sorgeloos (1983), respectively.
The latest hatching of your own cysts therefore the break up and you may line of the nauplii (instar-l to instar-III) was achieved according to the processes described inside Vanhaecke e al. (1983).
The types of nauplii were dehydrated from inside the an incubator in the 55°C having forty-eight hours. For each and every naupliar phase examined, 4-6 replicated examples, around 10 mg deceased thing was in fact burned totally inside sheer fresh air in addition to their caloric blogs is mentioned inside the a beneficial Phillipson microbomb calorimeter.
In advance of investigation, trials have been homogenized for the an enthusiastic ultrasonic homogenizer (Sonifier B12) and you will overall lipids had been extracted according to sort of Bilgh Dyer (1959). Saponification and you can methylation was performed once the described from the Shauer Simpson (1978). Greasy acid methylesters was basically inserted in the a beneficial capillary line (twenty five meters fused silica; interior diameter; 0.thirty two mm; liquide phase : SILAR 10C, movie density: 0.3um) hung into the an excellent Carlo Erba Mega 2350 gasoline chromatograph.
